Histone-like Nucleoid-Structuring Protein (H-NS) Paralogue StpA Activates the Type I-E CRISPR-Cas System against Natural Transformation in Escherichia coli
نویسندگان
چکیده
منابع مشابه
Nucleotide sequence of a newly-identified Escherichia coli gene, stpA, encoding an H-NS-like protein.
analysis of a 978-bp fragment of this segment revealed an ORF , w . r o , * r. A A , ^ c ,,noo> ^ * o i«> M.-> , J , r . b r IIA • J /i-.N 1Martinez,E., Bartolomi.B. and de la Cruz,F. (1988) Gene 68, 159-162. that encodes a putative protein of 134 ammo acids (Figure 1). 2 DevereuxJ., Haeberli.P. and Smithies.O. (1984) Nucleic Acids Res. 12, Comparative searches (FASTA, TFASTA (2)) showed that t...
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Pseudomonas aeruginosa contains two distinct members of H-NS family of nucleoid-structuring proteins: MvaT and MvaU. Together, these proteins bind to the same regions of the chromosome and function coordinately in the regulation of hundreds of genes. Due to their structural similarity, they can associate to form heteromeric complexes. These findings left us wondering whether they bear similar D...
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Nucleoid-associated proteins are bacterial proteins that are responsible for chromosomal DNA compaction and global gene regulation. One such protein is Escherichia coli Histone-like nucleoid structuring protein (H-NS) which functions as a global gene silencer. Whereas the DNA-binding mechanism of H-NS is well-characterized, its paralogue, StpA which is also able to silence genes is less underst...
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The histone-like nucleoid structuring protein (H-NS) has played a key role in shaping the evolution of Shigella spp., and provides the backdrop to the regulatory cascade that controls virulence by silencing many genes found on the large virulence plasmid. H-NS and its paralogue StpA are present in all four Shigella spp., but a second H-NS paralogue, Sfh, is found in the Shigella flexneri type s...
متن کاملRole of histone-like proteins H-NS and StpA in expression of virulence determinants of uropathogenic Escherichia coli.
The histone-like protein H-NS is a global regulator in Escherichia coli that has been intensively studied in nonpathogenic strains. However, no comprehensive study on the role of H-NS and its paralogue, StpA, in gene expression in pathogenic E. coli has been carried out so far. Here, we monitored the global effects of H-NS and StpA in a uropathogenic E. coli isolate by using DNA arrays. Express...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2020
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.00731-20